Lactophenol cotton blue definition biology


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Ringworm is a dermatophyte, meaning a fungus that feeds on keratin: skin, hair, nails, etc. More specifically, ringworm feeds on the outer layer of skin and hair follicles and this causes the hair to become brittle and break.

Diagnosis can be made several ways.

LABORATORY DIAGNOSIS OF FUNGI – HOW TO DIAGNOSE FUNGI IN LABS ?

Visually, ringworm often causes balding and ring shaped lesions on the skin. Some species will fluoresce green or blue under a woods lamp, but again, not guaranteed. Another diagnostic method is culturing skin and hair from the affected area. It is cultured in dextrose agar with phenol red and kept at room temperature for days. A positive culture will turn the agar red.

Ringworm can be very contagious and could spread to others in the time it takes for a positive culture. Ringworm can also be diagnosed by looking at a sample under a light microscope. This is the fastest, most reliable method. Some species of ringworm are species specific, whereas others are zoonotic. The three most common are : Trichophyton mentogrophytes, Microsporum canis, Microsporum gypseum and these are all zoonotic. Species are identified by the shape and number of cells in the macroconidium; a large spore that is produced through vegetative reproduction.

They grow on specialized hyphae until mature. All slides were prepared with a coverslip and sealed with clear nail polish. The accepted method for collecting spores is to take a piece of cellophane tape and lightly graze it over the culture. The tape is then placed a slide with any stain that will be used. I wanted to compare this with taking whole sections. I obtained sections by scraping cultures with a needle. I then scrambled the needle in a drop of stain or milipore water and applied the coverslip.

I also wanted to experiment with different stains. I compared Lactophenol cotton blue, which is a stain used with light microscopy. I also compared these to spores without stain and auto-fluorescence only.

I first looked at a slide prepared with a tape sample with lactophenol cotton blue. This did not fluoresce at all so I did not go any further with it, such as adding KOH. This is similar to what spores would look like under a light microscope, only with lactophenol, it would appear blue.

I am into fungi and I have a microscope and I happen to have come down with ringworm. Exciting, I know. But I can't seem to locate any spores on tape from my skin. How are spores collected for culture?

lactophenol cotton blue definition biology

Marine Biology. Electrical Engineering.In this article we will discuss about the m eaning and some m ethods of double staining.

lactophenol cotton blue definition biology

Staining is essential because different tissues and other structures can be easily differentiated by staining. The plant materials, in which there is no differentiation of tissues such as members of algae, fungi and bryophyta, are stained by a single staining process.

Aniline blue, fast-green, acetocarmine and haematoxylin are some of the other stains used for algal preparations. Cotton blue is supposed to be one of the good fungal stains. After staining with cotton blue, mount the material in lactophenol if the former is not prepared in the lactophenol.

Cotton blue serves as a staining as well as mounting medium if prepared in lactophenol. Aniline blue or haematoxylin also gives satisfactory staining results in certain cases.

See under the microscope that only thick-walled cells are stained. Now stain the material with few drops of fast green for few seconds. Time for keeping the material in fast green varies from few seconds to one minute for different materials.

With this method, all thick-walled cells get red stain and all thin-walled cells the green stain. Keep the sections in Delafield haematoxylin for four to five minutes and remove the excess of stain with water. Wash with ammonia. Now stain with safranin for few minutes.

Wash the sections with glycerine for removing excess of stain and mount in glycerine. BotanyPracticalsStainingMeaning of Staining.

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Top Menu BiologyDiscussion. Biology Permanent Slide Preparations: 3 Methods.

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Permanent Slide Preparations: 3 Methods. This is a question and answer forum for students, teachers and general visitors for exchanging articles, answers and notes. Answer Now and help others. Answer Now. Here's how it works: Anybody can ask a question Anybody can answer The best answers are voted up and rise to the top.Filamentous Identification Lab Service.

One reason to identify filaments is to determine the filaments characteristics and then determine the type present. If the type is found out, a root cause can usually be associated with a particular filament.

If the cause is known, then a correction can be made to alleviate problems. Chlorination is only a quick fix. Without process changes, filaments will grow back after chlorination. Training is an integral part of any job. Not everyone is at the same level of training. Many people want beginning concepts and basics. Some need technical information or troubleshooting. Some want equipment, technology or process information.

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Check out our new Services. We are in the process of developing new courses for our ""Online E-University" in order to meet the needs of our global customers that cannot travel to our public classes. Visit our new website www. Stains, what to use and where to find them: There are many companies that make stains for Microscopic work.

We are constantly asked what are the most common used for wastewater. Below are just some examples. Feel free to check your local supplier or check other sources off the Internet. These are just used as a guideline to some of the easy types of stains to use. Gram Stains and Neisser stains are more difficult to use and are for use with filamentous work. Identify microorganisms without mixing or measuring reagents and stains.

Each dropper contains a one-day supply of reagent or stain, hermetically sealed inside an ampoule. Strict quality controls assure uniformity and accuracy. To perform tests, squeeze the dropper to crush the ampoule. Invert the dropper for convenient drop-by-drop dispensing.

A clear dropper stand with cover is supplied to eliminate spills. Dispenser box blocks out light to prevent decomposition. USA Bluebook - www.

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Lactophenol Cotton Blue Stain is formulated with lactophenol, which serves as a mounting fluid, and cotton blue. Organisms suspended in the stain are killed due to the presence of phenol.Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising.

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Slide Culture and Lactophenol Cotton Blue Staining

We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime. Staining techniques. Upcoming SlideShare. Like this presentation? Why not share! Embed Size px. Start on. Show related SlideShares at end.

lactophenol cotton blue definition biology

WordPress Shortcode. Published in: Science. Full Name Comment goes here. Are you sure you want to Yes No. Kayeef Rahaman. Mir Mohsin. Apurva Sakhare. Vikram Lp. Show More. No Downloads. Views Total views. Actions Shares. No notes for slide. Staining techniques 1.

Dyes Basic dyes: Methylene blue, Basic fuchsin, Crystal violet, Safranine, Malachite green have positively charged groups usually from penta-valent nitrogen and are generally sold as chloride salts. Basic dyes bind to negatively charged molecules such as nucleic acids, many proteins and surfaces of bacterial and archeal cells.

Acidic dyes, in their ionized form, have a negative charge and bind to positively charged cell structures. Positive staining 5 6. Principle Negative staining requires the use of acidic stains such as Indian ink and nigrosin.The lactophenol cotton blue LPCB wet mount preparation is the most widely used method of staining and observing fungi and is simple to prepare.

The preparation has three components:.

Identification of fungi: criteria for identification of yeast and mold

Lactophenol cotton blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic examination of fungi. Fungal elements are stained intensely blue. Thoroughly mix them using a magnetic stirrer. You can also use ml of ethanol in the mixture if required.

I have a question to the staining agent Lactophenol Cotton Blue used for a urine sample — does anything else other than fungal structures bind to LPCB, mucus threads or anything else? This confused me, as they are not supposed to pick up the dye? Can anyone help? How long does the phenol cotton blue stain need to be in contact with a Fungus before it is safe to bring from under a hood to examine at microscopically?

This site uses Akismet to reduce spam. Learn how your comment data is processed. Culture Media Tests microbes Difference Between. News Ticker. About Acharya Tankeshwar Articles. Hello, thank you for visiting my blog. I am Tankeshwar Acharya. Blogging is my passion. I am working as an Asst. Next Atypical Mycobacterial Infections. Hello, I have a question to the staining agent Lactophenol Cotton Blue used for a urine sample — does anything else other than fungal structures bind to LPCB, mucus threads or anything else?

Can lacto phenol cotton blue stain we can used to demonstrate pneumocystis jiroveci??? Do you have any queries? Please leave me in the comments section below. I will be happy to read your comments and reply.She is passionate about education, writing, and travel. You should definitely follow us on social media.

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Current and Next Game Odd or Even - In the event of a game starting but not being completed then all bets will be void. Current and Next Game Winning Margin - In the event of a game starting but not being completed then all bets will be void. The below markets are based on a statutory number of games being played.

In the event of any of the named players in a match changing before the match starts then all bets are void. In-Play Game Markets (Current and Next) - The specified game must be completed for bets to stand, unless the specific market outcome is already determined.

In-Play Race to Markets - Bets are settled based on the first player to reach the nominated number of points in the relevant game. In the event of neither player reaching the number of points required (because of abandonment) then bets on that market will be void. If the relevant game is not played then all race markets for that game will be void. In-Play Point Betting - Bets are offered for a player to win the nominated point. Total Point Betting markets are based on the statutory number of games being played.

In-Play Handicap Betting - Markets are based on the statutory number of games being played. Both players in a specified match-up must play 1 point in the tournament for bets to stand. If players progress to the same round of the tournament bets will be void. In the event of the first set not being completed bets will be void, unless the score has reached 6-6 in which case the market will be settled as Yes.

In the event of the match starting but not being completed all bets are void unless a tie-break has already occurred, or it is impossible for a tie-break to occur e. In the event of match starting but not being completed all bets are void. Both 2-Way and 3-Way markets may be offered. Bets are void if the statutory number of sets are not completed, or are changed.

In the event of a disqualification or retirement all bets will be void, unless settlement of the bet has already been determined. Bets settled from official tournament statistics. The 1st Service Game must be completed in full for bets to stand. Name the first player to break serve in the match. If there is no break of serve in the match, bets will be settled as a push.

In-Play bets on 3, 4 and 5 Sets respectively are void as the outcome of the market cannot be unconditionally determined. The Handicap, Total Games In Match and Player Games markets are based on a statutory number of sets (see related Set Betting). In the event of the statutory number of sets being changed or differing from those offered for betting purposes then all bets are void.

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If no next set played then bets on that set will be void. The nominated set must be completed for bets to stand.


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